Roles of interstitial fluid pH in diabetes mellitus: Glycolysis and mitochondrial function

Figure 5 Effects of extracellular pH on the expression of insulin receptor on the plasma membrane. After serum starved for 4 h, L6 myotubes were treated with 100 nmol/L insulin for 15 min in the HEPES buffer with different pH. Proteins expressed on the plasma membrane were biotinylated and precipitated. A: Representative blots of total expression of insulin receptor on the plasma membrane, the Na⁺, K⁺-ATPase, and GAPDH; B: Quantative data of expression of insulin receptor on the plasma membrane at different pH normalized to that at pH 7.4. The results are presented as the mean ± SEM (n = 8). pH had no significant effects on the expression of insulin receptor on the plasma membrane. Modified from ref.[46] with allowance of non-profit use of figures.