Ginsenoside Rd promote the contact between mitochondria and lipid droplets through Plin1/Mfn2 to treat diabetic kidney disease

Figure 9 Ginsenoside Rd enhances the coupling of mitochondria and lipid droplets via perilipin 1-mitofusin 2 signaling pathway in HK-2 cells. A: Western blot bands of fibronectin, collagen I and α-smooth muscle actin; B: Western blot analysis of fibronectin (n = 4); C: Western blot analysis of collagen I (n = 4); D: Western blot analysis of α-smooth muscle actin (n = 4); E: Triglycerides in blood serum (n = 3); F: Free fatty acid in blood serum (n = 3); G: The polymerase chain reaction (PCR) results of hydroxyacyl-CoA dehydrogenase trifunctional multienzyme complex subunit beta (n = 3); H: The PCR results of carnitine O-octanoyltransferase (n = 3); I: The PCR results of medium-chain acyl-CoA dehydrogenase (n = 3); J: The PCR results of perilipin (Plin) 1 (n = 3); K: The PCR results of mitofusin (Mfn) 2 (n = 3); L: Western blot bands of protein kinase A, Plin1 and Mfn2; M: Western blot analysis of protein kinase A (n = 4); N: Western blot analysis of Plin1 (n = 4); O: Western blot analysis of Mfn2 (n = 4). Data are presented as mean ± SEM. ^aP < 0.01 compared with control group; ^bP < 0.01 compared with model group; ^cP < 0.01 compared with Rd group. PA: Palmitic acid; SMA: Smooth muscle actin; HADHB: Hydroxyacyl-CoA dehydrogenase trifunctional multienzyme complex subunit beta; CROT: Carnitine O-octanoyltransferase; MCAD: Medium-chain acyl-CoA dehydrogenase; FFA: Free fatty acid; Plin1: Perilipin 1; Mfn2: Mitofusin 2.