Ginsenoside Rd promote the contact between mitochondria and lipid droplets through Plin1/Mfn2 to treat diabetic kidney disease

Figure 8 CCK-8 and siRNA in HK-2 cells. A: HK-2 experimental protocol; B: The CCK-8 of palmitic acid (n = 4); C: The CCK-8 of Rd (n = 4); D: Knockdown efficiency of si-perilipin (Plin) 1 at mRNA level (n = 3); E: Western blot bands of si-Plin1; F: Knockdown efficiency of si-Plin1 at protein level (n = 3); G: Co-immunoprecipitation analysis of the interaction between Plin1 and mitofusin 2 (n = 3). Data are presented as mean ± SEM. ^aP < 0.05 compared with control group; ^bP < 0.05, ^cP < 0.01 compared with negative control siRNA group. Plin1: Perilipin 1; Mfn2: Mitofusin 2; PA: Palmitic acid; IgG: Immunoglobulin G; siRNA-NC: Negative control siRNA.