Plasma metabolomics reveals progressive alterations across glycemic states

Figure 2 Differential metabolite screening and functional enrichment analysis. A: Variable importance analysis of differential metabolites in the overall study population based on the random forest model; B: UpSet plot showing the intersections of differential metabolites identified in the comparisons between the normal glucose tolerance (NGT) and isolated impaired fasting glucose groups (A vs B), the NGT and isolated impaired glucose tolerance groups (A vs C), and the NGT and diabetes groups (A vs D); C: Heatmap showing the expression patterns of the 18 shared differential metabolites across the four groups (NGT, impaired fasting glucose, impaired glucose tolerance, and diabetes); D: Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis based on the 18 shared differential metabolites. The X-axis represents the enrichment ratio, bubble size indicates the number of metabolites enriched in each pathway, and bubble color represents the adjusted P value.