Electroacupuncture alleviates diabetic peripheral neuropathy: Regulates mitochondrial biogenesis and redistribution in peripheral nerve fibers

Figure 7 Selisistat injection decreased mitochondrial levels at nodes of Ranvier but increased mitochondrial levels in intraepidermal nerve fibers. A-C: Mitochondrial levels at nodes of Ranvier were assessed by representative immunofluorescence images of pyruvate dehydrogenase (PDH)-stained mitochondria (red) at nodes of Ranvier marked by voltage-gated sodium channel 1.6 (green) in sciatic nerve fibers with axonal diameters of 5 μm (A) and 2 μm (B), and quantification of relative mean fluorescence intensity (C). The white dotted frames indicate the positions of nodes of Ranvier; D and E: Mitochondrial levels in intraepidermal nerve fibers were assessed by representative immunofluorescence images of PDH-stained mitochondria (red) in intraepidermal nerve fibers marked by protein gene product 9.5 (green) (D), and quantification of relative mean fluorescence intensity (E). ^fP < 0.01 electroacupuncture plus selisistat group vs electroacupuncture group. EA: Electroacupuncture; EA + E: Electroacupuncture plus selisistat; PDH: Pyruvate dehydrogenase.