Assessment of the role of general control nonderepressible 2 in gestational diabetes mellitus progression and the underlying mechanisms

Figure 7 General control nonderepressible 2 absence inactivated endoplasmic reticulum stress to retard the apoptosis and oxidative damage in high glucose-exposed HTR-8/SVneo cells. A: Reactive oxygen species production assessed by 2′,7′-dichlorodihydrofluorescein diacetate staining; B: Oxidative stress-related indices including malondialdehyde, superoxide dismutase and catalase; C: Apoptosis level determined by flow cytometry; D: Expression levels of apoptosis-related proteins. ^aP < 0.001 vs normal glucose; ^bP < 0.001 vs high glucose (HG) + sh-negative control; ^cP < 0.05 vs HG + sh-GCN2; ^dP < 0.01 vs HG + sh-GCN2; ^eP < 0.001 vs HG + sh-GCN2. NG: Normal glucose; HG: High glucose; MDA: Malondialdehyde; NC: Negative control; GCN2: General control nonderepressible 2; TM: Tunicamycin; SOD: Superoxide dismutase; CAT: Catalase.