Assessment of the role of general control nonderepressible 2 in gestational diabetes mellitus progression and the underlying mechanisms

Figure 6 General control nonderepressible 2 absence inactivated endoplasmic reticulum stress to mitigate mitochondrial dysfunction in high glucose-exposed HTR-8/SVneo cells. A and B: General control nonderepressible 2 (GCN2) expression at mRNA and protein levels determined by reverse transcription quantitative PCR (RT-qPCR) and Western blotting; C: Expression levels of endoplasmic reticulum stress-related proteins; D: Mitochondrial membrane potential assessed by JC-1 staining; E: ATP content in cells; F: The mtDNA content determined by RT-qPCR. ^aP < 0.001 vs normal glucose; ^bP < 0.01 vs high glucose (HG) + sh-negative control (NC); ^cP < 0.001 vs HG + sh-NC; ^dP < 0.001 vs HG + sh-GCN2. GCN2: General control nonderepressible 2; GDM: Gestational diabetes mellitus; NC: Negative control; eIF2α: Eukaryotic initiation factor 2α; NG: Normal glucose; HG: High glucose.