Assessment of the role of general control nonderepressible 2 in gestational diabetes mellitus progression and the underlying mechanisms

Figure 4 General control nonderepressible 2 knockdown attenuated apoptosis and oxidative stress in placental tissues of gestational diabetes mellitus mice. A: Histological changes in placental tissues; B: Apoptosis level in placental tissues as detected by terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling staining; C: Expression levels of apoptosis-related proteins determined by Western blotting; D: Reactive oxygen species production assessed by dihydroethidium staining; E: Oxidative stress-related indices including malondialdehyde, superoxide dismutase, and catalase. ^aP < 0.001 vs control; ^bP < 0.01 vs gestational diabetes mellitus (GDM) + sh-negative control (NC); ^cP < 0.001 vs GDM + sh-NC. GCN2: General control nonderepressible 2; GDM: Gestational diabetes mellitus; NC: Negative control; MDA: Malondialdehyde; SOD: Superoxide dismutase; CAT: Catalase.