Assessment of the role of general control nonderepressible 2 in gestational diabetes mellitus progression and the underlying mechanisms

Figure 1 General control nonderepressible 2 is upregulated in placental tissues of gestational diabetes mellitus mice. A and B: Reverse transcription quantitative PCR (RT-qPCR; A) and Western blotting analysis (B) of general control nonderepressible 2 (GCN2) expression; C and D: RT-qPCR (C) and Western blotting analysis (D) of GCN2 knockdown efficiency; E and F: RT-qPCR (E) and Western blotting (F) analysis of GCN2 expression in placental tissues of gestational diabetes mellitus (GDM) mice. ^aP < 0.001 vs control; ^bP < 0.001 vs sh-negative control (NC); ^cP < 0.001 vs GDM + sh-NC. GCN2: General control nonderepressible 2; GDM: Gestational diabetes mellitus; NC: Negative control.