Shenqi Jiangtang Granules attenuate ferroptosis in diabetic cardiomyopathy via the dihydroorotate dehydrogenase-coenzyme Q pathway

Figure 7 Shenqi Jiangtang Granules-containing serum modulates the dihydroorotate dehydrogenase/coenzyme Q signaling pathway and mitochondrial morphology in H9C2 cells under glucolipotoxicity. A: Reverse transcription-quantitative PCR analysis of ferroptosis-related marker genes and dihydroorotate dehydrogenase (DHODH)/coenzyme Q10A pathway genes; B and C: Western blot analysis of ferroptosis-related marker proteins and DHODH/coenzyme Q pathway proteins, with densitometric quantification; D: Confocal microscopy of mitochondrial morphology following MitoTracker staining (n = 3). ^aP < 0.05 vs control; ^bP < 0.01 vs control; ^cP < 0.05 vs model group; ^dP < 0.01 vs model group; ^eP < 0.01 vs Shenqi Jiangtang Granules-high + brequinar. Con: Control; Mod: Model; SQJT: Shenqi Jiangtang Granules; SQJT-H: Shenqi Jiangtang Granules-high; BRQ: Brequinar.