Shenqi Jiangtang Granules attenuate ferroptosis in diabetic cardiomyopathy via the dihydroorotate dehydrogenase-coenzyme Q pathway

Figure 6 Shenqi Jiangtang Granules enhance antioxidant defense of H9C2 cells under glucolipotoxicity. A: Intracellular free iron quantified by colorimetric assay; B: Malondialdehyde (MDA), the end product of lipid peroxidation, measured using the thiobarbituric acid-MDA condensation assay; C: Superoxide dismutase activity quantified by the WST-1 method; D: Glutathione peroxidase activity quantified by colorimetric assay; E: Glutathione quantified by the 5,5′-dithiobis-(2-nitrobenzoic acid) colorimetric/spectrophotometric assay; F and G: Flow cytometric detection of reactive oxygen species-positive cells using fluorescent probes (n = 3). ^aP < 0.05 vs control; ^bP < 0.01 vs control; ^cP < 0.05 vs model group; ^dP < 0.01 vs model group; ^eP < 0.05 vs Shenqi Jiangtang Granules-high (SQJT-H) + brequinar (BRQ); ^fP < 0.01 vs SQJT-H + BRQ. Con: Control; Mod: Model; SQJT: Shenqi Jiangtang Granules; MDA: Malondialdehyde; SOD: Superoxide dismutase; GSH: Glutathione; GSH-Px: Glutathione peroxidase; ROS: Reactive oxygen species.