Shenqi Jiangtang Granules attenuate ferroptosis in diabetic cardiomyopathy via the dihydroorotate dehydrogenase-coenzyme Q pathway

Figure 5 Shenqi Jiangtang Granules significantly attenuate H9C2 cell injury. A: CCK-8 assay showing the effects of high glucose combined with different concentrations of palmitic acid (PA) on H9C2 cells (0-0.5 mmol/L); B: Effects of high glucose combined with 0.45 mmol/L PA for 6-, 12-, and 24-hour on H9C2 cell injury; C: Dose-dependent protective effects of Shenqi Jiangtang Granules (SQJT)-containing serum against glucolipotoxicity-induced H9C2 cell injury. Low-, medium-, and high-dose groups correspond to 2%, 4%, and 6%, respectively; D and E: Flow cytometry analysis of propidium iodide-positive cells (n = 3). ^aP < 0.05 vs control; ^bP < 0.01 vs control; ^cP < 0.05 vs model group; ^dP < 0.01 vs model group; ^eP < 0.01 vs SQJT-high + brequinar; ^fP < 0.01 vs 6 hours control; ^gP < 0.01 vs 12 hours control; ^hP < 0.01 vs 24 hours control.