Network pharmacology: Anti-obesity and insulin-sensitizing effects of Danggui-Huangjing herb-pair via epidermal growth factor receptor/phosphatidylinositol 3-kinase/protein kinase B pathway

Figure 8 Danggui-Huangjing robustly reduces the expression of inflammation and lipogenesis-related genes in obese C57BL/6 mice. A: Relative hepatic mRNA expression of IL6; B: Relative hepatic mRNA expression of IL1b; C: Relative hepatic mRNA expression of Tnfa; D: Relative hepatic mRNA expression of Cd11b; E: Relative hepatic mRNA expression of Nos2; F: Relative hepatic mRNA expression of Pi3k; G: Relative hepatic mRNA expression of Akt; H: Relative hepatic mRNA expression of Fasn; I: Relative hepatic mRNA expression of Egfr; J: Relative hepatic mRNA expression of Stat3; K: Relative hepatic mRNA expression of Acc; L: Relative hepatic mRNA expression of Srebp1. RNA extraction (AG21017 kit), complementary DNA synthesis (AG11728 kit), qPCR (StepOne Plus system, SYBR Green kit AG11739); β-Actin as internal reference, relative expression via 2^-ΔΔCt method. Data are presented as mean ± SD and were analyzed by one-way analysis of variance followed by the Bonferroni post hoc test (n = 6/group). ^aP < 0.05, ^bP < 0.01, ^cP < 0.001 vs normal control group; ^dP < 0.05, ^eP < 0.01, ^fP < 0.001 vs model control group; ^gP < 0.05, ^hP < 0.01, ⁱP < 0.001 vs Danggui-Huangjing group. NS: Not significant (for Danggui-Huangjing vs Danggui/Huangjing groups, no statistical difference). DG: Danggui; DH: Danggui-Huangjing; HJ: Huangjing; MC: Model control; NC: Normal control.