Network pharmacology: Anti-obesity and insulin-sensitizing effects of Danggui-Huangjing herb-pair via epidermal growth factor receptor/phosphatidylinositol 3-kinase/protein kinase B pathway

Figure 7 Danggui-Huangjing alleviates adipose inflammation and hepatic steatosis in obese C57BL/6 mice. A: Representative micrographs (200 ×): Hematoxylin and eosin/Oil Red O-stained sections (processing as Figure 6A); B-F: Serum interleukin-6, interleukin-1β, tumor necrosis factor-α, leptin, adiponectin (n = 6/group), measured by enzyme-linked immunosorbent assay kits (Ruixin Biotechnology) with E9032 microplate reader (duplicate assays). Data are presented as mean ± SD and were analyzed by one-way analysis of variance followed by the Bonferroni post hoc test. ^aP < 0.05, ^bP < 0.01, ^cP < 0.001 vs normal control group; ^dP < 0.05, ^eP < 0.01, ^fP < 0.001 vs model control group; ^gP < 0.05, ^hP < 0.01, ⁱP < 0.001 vs Danggui-Huangjing group. NS: Not significant (for Danggui-Huangjing vs Danggui/Huangjing groups, no statistical difference). BAT: Brown adipose tissue; DG: Danggui; DH: Danggui-Huangjing; HJ: Huangjing; IL: Interleukin; MC: Model control; NC: Normal control; TNF-α: Tumor necrosis factor-α; WAT: White adipose tissue.