Network pharmacology: Anti-obesity and insulin-sensitizing effects of Danggui-Huangjing herb-pair via epidermal growth factor receptor/phosphatidylinositol 3-kinase/protein kinase B pathway

doi:10.4239/wjd.v17.i5.118275

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World Journal of Diabetes

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1948-9358

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Copyright: ©Author(s) 2026. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution-NonCommercial (CC BY-NC 4.0) license. No commercial re-use. See permissions. Published by Baishideng Publishing Group Inc.

World J Diabetes. May 15, 2026; 17(5): 118275
Published online May 15, 2026. doi: 10.4239/wjd.v17.i5.118275

Table 1 Primers used for real-time quantitative polymerase chain reaction

Primer	Sequence (5’-3’)	Length (bp)
actin	Forward primer: TGTACCCAGGCATTGCTGAC	238
actin	Reverse primer: AACGCAGCTCAGTAACAGTCC	238
Egfr	Forward primer: CCTATGCCACGCCAACTGTA	90
Egfr	Reverse primer: CACTGCCATTGAACGTACCC	90
Pi3k	Forward primer: CGGAGAACCTATTGCGAGGG	106
Pi3k	Reverse primer: TGCTTGACTTCGCCGTCTAC	106
Akt	Forward primer: CCTTTATTGGCTACAAGGAACGG	170
Akt	Reverse primer: CACATGGAAGGTGCGCTCAA	170
Fasn	Forward primer: CAAGCAGGCACACACAATGG	176
Fasn	Reverse primer: GAAGCGTCTCGGGATCTCTG	176
Srebp1	Forward primer: CGGCTGCATTGAGAGTGAAG	201
Srebp1	Reverse primer: CTGCTCAGGAAGAAGCGTGTC	201
Acc	Forward primer: TCGGCACATGGAGATGTACG	119
Acc	Reverse primer: GATCTACCCGACGCATGGTT	119
Stat3	Forward primer: GCCAAATGCTTGGGCATCAA	92
Stat3	Reverse primer: AGGTTCCAATTGGCGGCTTA	92
IL6	Forward primer: CGGCCTTCCCTACTTCACAA	184
IL6	Reverse primer: GCAAGTGCATCATCGTTGTTC	184
IL1b	Forward primer: AATGAAAGACGGCACACCCA	119
IL1b	Reverse primer: ACTCCACTTTGCTCTTGACTTCT	119
Tnfa	Forward primer: ACTCCAGGCGGTGCCTATGT	160
Tnfa	Reverse primer: GTGAGGGTCTGGGCCATAGAA	160
Cd11b	Forward primer: GCACCACACATGCAGAGAGA	151
Cd11b	Reverse primer: GCTACCCGAGCCATCAATCA	151
Nos2	Forward primer: ACTACTGCTGGTGGTGACAA	106
Nos2	Reverse primer: GAAGGTGTGGTTGAGTTCTCTAAG	106

Full Size Table

Citation: Li WJ, Xu JJ, Tiang HS, Liu TH, Wu LL. Network pharmacology: Anti-obesity and insulin-sensitizing effects of Danggui-Huangjing herb-pair via epidermal growth factor receptor/phosphatidylinositol 3-kinase/protein kinase B pathway. World J Diabetes 2026; 17(5): 118275
URL: https://www.wjgnet.com/1948-9358/full/v17/i5/118275.htm
DOI: https://dx.doi.org/10.4239/wjd.v17.i5.118275

Li WJ, Xu JJ, Tiang HS, Liu TH, Wu LL. Network pharmacology: Anti-obesity and insulin-sensitizing effects of Danggui-Huangjing herb-pair via epidermal growth factor receptor/phosphatidylinositol 3-kinase/protein kinase B pathway. World J Diabetes 2026; 17(5): 118275 [DOI: 10.4239/wjd.v17.i5.118275]

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