Gastric electrical stimulation ameliorates antral myenteric neurons in streptozotocin-induced diabetic beagle canines

Figure 6 Representative immunofluorescence images of myenteric calretinin-immunoreactive positive neurons in full-thickness sections of canine gastric antrum (200 × magnification, scale bar = 50 μm). A: Representative images of anti-human neuronal protein HuC/HuD-immunoreactive (anti-Hu-IR) labeled neurons in diabetes mellitus (DM) + gastric electrical stimulation (GES); B: Representative images of calretinin-immunoreactive (CALR-IR) positive neurons in DM + GES; C: Representative merged images of anti-Hu-IR/CALR-IR in DM + GES; D: Representative images of anti-Hu-IR labeled neurons in DM + SGES; E: Representative images of CALR-IR positive neurons in DM + SGES; F: Representative merged images of anti-Hu-IR/CALR-IR in DM + SGES; G: Representative images of anti-Hu-IR labeled neurons in control + GES; H: Representative images of CALR-IR positive neurons in control + GES; I: Representative merged images of anti-Hu-IR/CALR-IR in control + GES; J: Representative images of anti-Hu-IR labeled neurons in control + SGES; K: Representative images of CALR-IR positive neurons in control + SGES; L: Representative merged images of anti-Hu-IR/CALR-IR in control + SGES; M: Number of anti-Hu-IR positive neurons; N: Number of CALR-IR positive neurons; O: Proportion of CALR-IR positive neurons relative to total neurons. Anti-Hu: Anti-human neuronal protein HuC/HuD; CALR: Calretinin; DM: Diabetes mellitus; GES: Gastric electrical stimulation; IR: Immunoreactive; SGES: Sham gastric electrical stimulation.