EP300 drives renal fibrosis in diabetic nephropathy via histone acetyltransferase-mediated nephrocystin-4 expression

Figure 6 Inhibition of the EP300-nephrocystin-4 axis affects renal fibrosis markers in HK-2 cells. A-C: Representative western blot images and quantitative analysis of alpha-smooth muscle actin and fibronectin protein levels in nephrocystin-4-knockdown cells; D-F: Representative western blot images and quantitative analysis of alpha-smooth muscle actin and fibronectin protein levels in nephrocystin-4-overexpressing cells. Data are presented as mean ± SD (n = 3). ^aP < 0.05, ^bP < 0.01, and ^cP < 0.001. α-SMA: Alpha-smooth muscle actin; HG: High glucose; NPHP4-OE: Overexpression of nephrocystin-4; siNPHP4: Small interfering RNA targeting nephrocystin-4.