EP300 drives renal fibrosis in diabetic nephropathy via histone acetyltransferase-mediated nephrocystin-4 expression

Figure 3 Effect of nephrocystin-4 on HK-2 cell proliferation and apoptosis. A and B: Measurement of nephrocystin-4 knockdown and overexpression efficiency in HK-2 cells by real-time quantitative polymerase chain reaction; C: Measurement of HK-2 cell viability by cell counting kit-8 assay; D: Measurement of HK-2 cell apoptosis by TUNEL staining. Scale bar = 20 µm. Data are presented as mean ± SD (n = 3). ^bP < 0.01, ^cP < 0.001. HG: High glucose; NPHP4-OE: Overexpression of nephrocystin-4; siNPHP4: Small interfering RNA targeting nephrocystin-4.