Hyperoside attenuates diabetic nephropathy by activating the Nrf2/SLC7A11/GPX4 axis to restrain ferroptosis

Figure 1 Effects of hyperoside on HK-2 cell viability under high-glucose conditions. ^cP < 0.001, ^dP < 0.001. One-way analyses of variance with Tukey’s post hoc test; two-sided. Data are expressed as the mean ± SD; n = 3 independent experiments. A: Cell Counting Kit-8 assay showing viability with hyperoside (1 μM, 10 μM, 50 μM, and 100 μM) under high glucose (HG) (30 mmol/L); B: Terminal deoxynucleotidyl transferase dUTP nick-end labeling: Normal glucose, HG, and HG + hyperoside (50 μM); quantification of terminal deoxynucleotidyl transferase dUTP nick-end labeling-positive cells; C: Flow cytometry apoptosis rates in the same groups. NG: Normal glucose; HG: High glucose; DAPI: 4’,6-diamidino-2-phenylindole; TUNEL: Terminal deoxynucleotidyl transferase dUTP nick-end labeling; FITC: Fluorescein isothiocyanate.