C1q tumor necrosis factor associated protein 3 protects HaCaT via phosphoinositide 3-kinase/protein kinase B activation by PTEN reduction

Figure 5 PTEN reversed the protective effects of C1q tumor necrosis factor associated protein 3 on HaCaT cells. A: Quantitative polymerase chain reaction (qPCR) analysis of messenger RNA (mRNA) levels of PTEN in HaCaT cells before and after transfection; B: Cell viability of HaCaT cells before and after transfection is detected by cell counting kit-8 assay; C and D: Representative images and quantitative analysis of HaCaT cells before and after transfection were measured by 5-ethynyl-2’-deoxyuridine method to obtain the cell growth; E and F: Cell migration of HaCaT cells before and after transfection was detected by transwell method; G and H: Representative images and quantitative analysis of HaCaT cells before and after transfection were measured by scratch wound healing method to obtain the cell migration; I: QPCR analysis of mRNA levels of vascular endothelial growth factor in HaCaT cells before and after transfection; J: Representative images of key proteins of phosphoinositide 3-kinase/protein kinase B pathway in HaCaT cells before and after transfection. ^bP < 0.01 vs vector group. ^dP < 0.0001 vs 5.6 mmol/L glucose group. ^hP < 0.0001 vs 30 mmol/L glucose + vector group. ^jP < 0.01 vs 30 mmol/L glucose + C1q tumor necrosis factor associated protein 3 + vector group. ^kP < 0.001 vs 30 mmol/L glucose + C1q tumor necrosis factor associated protein 3 + vector group. ^lP < 0.0001 vs 30 mmol/L glucose + C1q tumor necrosis factor associated protein 3 + vector group. mRNA: Messenger RNA; CTRP3: C1q tumor necrosis factor associated protein 3; NG: 5.6 mmol/L glucose group; HG: 30 mmol/L glucose group; DAPI: 4’,6-diamidino-2-phenylindole; EdU: 5-ethynyl-2’-deoxyuridine; PI3K: Phosphoinositide 3-kinase; p-PI3K: Phosphor-phosphoinositide 3-kinase; p-AKT: Phosphor-protein kinase B; AKT: Protein kinase B; GAPDH: Glyceraldehyde-3-phosphate dehydrogenase; VEGF: Vascular endothelial growth factor.