C1q tumor necrosis factor associated protein 3 protects HaCaT via phosphoinositide 3-kinase/protein kinase B activation by PTEN reduction

Figure 2 Overexpression of C1q tumor necrosis factor associated protein 3 ameliorates the detrimental effects of high glucose on the function of keratinocytes. A: Quantitative polymerase chain reaction (qPCR) analysis of messenger RNA (mRNA) levels of C1q tumor necrosis factor associated protein 3 in HaCaT cells before and after transfection; B: Cell viability of HaCaT cells before and after transfection is detected by cell counting kit-8 assay; C and D: Representative images and quantitative analysis of HaCaT cells before and after transfection were measured by 5-ethynyl-2’-deoxyuridine method to obtain the cell growth; E: Cell migration of HaCaT cells before and after transfection was detected by transwell method; F: Representative images and quantitative analysis of HaCaT cells before and after transfection were measured by scratch wound healing method to obtain the cell migration; G: QPCR analysis of mRNA levels of vascular endothelial growth factor in HaCaT cells before and after transfection. ^bP < 0.01 vs vector group. ^cP < 0.001 vs 5.6 mmol/L glucose group. ^dP < 0.0001 vs 5.6 mmol/L glucose group. ^fP < 0.01 vs 30 mmol/L glucose + vector group. ^gP < 0.001 vs 30 mmol/L glucose + vector group. ^hP < 0.0001 vs 30 mmol/L glucose + vector group. mRNA: Messenger RNA; CTRP3: C1q tumor necrosis factor associated protein 3; NG: 5.6 mmol/L glucose group; HG: 30 mmol/L glucose group; DAPI: 4’,6-diamidino-2-phenylindole; EdU: 5-ethynyl-2’-deoxyuridine; VEGF: Vascular endothelial growth factor.