C1q tumor necrosis factor associated protein 3 protects HaCaT via phosphoinositide 3-kinase/protein kinase B activation by PTEN reduction

Figure 1 C1q tumor necrosis factor associated protein 3 expression is low in the high-glucose-treated HaCaT cells. A: Quantitative polymerase chain reaction (qPCR) analysis of messenger RNA (mRNA) levels of C1q tumor necrosis factor associated protein 3 (CTRP3) in blood samples collected from normal and diabetic mice at days 0, 4, 8, 12, and 16; B: Western blot analysis of protein levels of CTRP3 in blood samples collected from normal and diabetic mice at days 0, 4, 8, 12, and 16; C: QPCR analysis of mRNA levels of CTRP3 in HaCaT cells treated with 5.6 mmol/L glucose or 30 mmol/L glucose 48 hours later; D: Western blot analysis of protein levels of CTRP3 in HaCaT cells treated with 5.6 mmol/L glucose or 30 mmol/L glucose 48 hours later. ^bP < 0.01 vs 5.6 mmol/L glucose group. ^dP < 0.0001 vs normal group. mRNA: Messenger RNA; CTRP3: C1q tumor necrosis factor associated protein 3; NG: 5.6 mmol/L glucose group; HG: 30 mmol/L glucose group; GAPDH: Glyceraldehyde-3-phosphate dehydrogenase.