PRDX2 silencing alleviates reactive hyperplasia of Müller glial cells in diabetic retinopathy by inhibiting the RhoA/ROCK signaling pathway

Figure 6 PRDX2 silencing inhibits RhoA/ROCK signaling in Müller cells. A: Tandem mass tagging proteomics of PRDX family proteins, RhoA, RhoGDI1, nestin in shPRDX2 and shNC groups; B and C: Protein expression of PRDX2, RhoA, ROCK1/2, and RhoGDI1 between shPRDX2 and shNC groups after high glucose- or normal glucose-treated rMC-1 cells for 48 hours by western blotting; D-F: The bar histograms represent measurements of the target protein's gray values. All values are presented as means ± SEM. Each experiment was independently repeated three times. P for trends assessed using the t test or ordinary one-way analysis of variance. ^aP < 0.05; ^bP < 0.01; ^cP < 0.001; ^dP < 0.0001. TMT: Tandem mass tag; NG: Normal glucose; HG: High glucose.