PRDX2 silencing alleviates reactive hyperplasia of Müller glial cells in diabetic retinopathy by inhibiting the RhoA/ROCK signaling pathway

Figure 4 PPDX2 silencing attenuates high glucose-induced Müller cell proliferation. rMC-1 cells transfected with control (shNC) or PRDX2 shRNA (shPRDX2) before being subjected to high glucose or normal glucose for 48 hours. A: mRNA expression of PRDX2 measured by quantitative polymerase chain reaction in wild-type, shNC, and shPRDX2 rMC-1 cells; B and C: Western blotting analysis of PRDX2 in rMC-1 cells. The bar histogram is representative of measurements of the gray values of PRDX2/β-actin (C); D and E: Hoechst staining was used to measure the total cell number (magnification: 100 ×; scale bars: 100 μm; blue: Hoechst); F: Cell viability as measured by CCK8 assay; G: Cell proliferation was measured by the CyQUANT NF cell proliferation assay kit; H and I: Cell proliferation detected by EdU staining (magnification: 200 ×; scale bars: 200 μm; red: EdU; blue: Hoechst). Bar histogram represents the quantitative results of rMC-1 cell proliferation rate under EdU staining (H). All values are presented as means ± SEM. Each experiment was independently repeated 3-5 times. P value for trends assessed using ordinary one-way analysis of variance. ^aP < 0.05; ^bP < 0.01; ^cP < 0.001; ^dP < 0.0001. WT: Wild type; NG: Normal glucose; HG: High glucose.