Fibroblast growth factor 1 alleviates diabetic nephropathy by reducing renal lipid accumulation in diabetic kidney

Figure 5 Fibroblast growth factor 1 attenuates fibrosis, inflammation, and lipid accumulation in high glucose and palmitate acid-treated human kidney-2 cells. A-C: The mRNA expression of collagen IV, interleukin-1β, and interleukin-6 was analyzed by RT-qPCR; D: Triglyceride content measured to assess lipid accumulation in high glucose and palmitate acid-treated human kidney-2 cells with or without fibroblast growth factor 1; E: Representative images of Oil Red O staining; F: Quantification of Oil Red O staining; G: Adipose differentiation related protein (ADRP) expression detected by immunofluorescence; H: Quantification of ADRP immunofluorescence; I: The mRNA expression of ADRP was analyzed by RT-qPCR; J: ADRP protein expression levels detected by Western blot; K: Densitometric analysis of Western blot using ImageQuant. Three to four independent experiments were performed for each analysis. Data were presented as mean ± SD. ^aP < 0.05. Bar = 20 μm. ADRP: Adipose differentiation related protein; Ctrl: Control; FGF1: Fibroblast growth factor 1; IL: Interleukin; HGPA: High glucose and palmitic acid.