Fibroblast growth factor 1 alleviates diabetic nephropathy by reducing renal lipid accumulation in diabetic kidney

Figure 2 Fibroblast growth factor 1 ameliorates diabetes-induced renal dysfunction and histopathological alterations. A: Urinary albumin-to-creatinine ratio for assessing renal function; B: Representative images of hematoxylin and eosin staining of kidney tissue and quantification of glomerular sizes based on hematoxylin and eosin staining; C: Periodic acid-Schiff staining and quantification of Periodic acid-Schiff positive staining area; D: Masson trichrome staining and quantification of Masson positive staining area; E: Sirius Red staining and quantification of Sirius Red positive staining area; F: F4/80 immunofluorescence staining and quantification of F4/80 positive macrophage; G-I: The mRNA expression levels of collagen IV, interleukin-1β, and interleukin-6 analyzed by RT-qPCR. Data were presented as mean ± SD. ^aP < 0.05. n = 6 mice per group. Bar = 200 μm. Ctrl: Control; FGF1: Fibroblast growth factor 1; HE: Hematoxylin and eosin; IL: Interleukin; PAS: Periodic acid-Schiff; T2DM: Type 2 diabetes mellitus.