Microtubule affinity-regulating kinase 4 exacerbates diabetic cardiomyopathy by inhibiting UNC-51-like kinase 1-mediated mitochondrial autophagy

Figure 5 Transcriptomic results and verification of the effects of microtubule affinity-regulating kinase 4 knockdown. A: Downregulated genes between the control + small hairpin (sh)-negative control (NC) and high glucose (HG) + sh-NC groups and upregulated genes between the HG + sh-NC and HG + sh-microtubule affinity-regulating kinase 4 groups are shown as a cross-Venn diagram; B: Kyoto Encyclopedia of Genes and Genomes classification of differentially expressed genes; C: Kyoto Encyclopedia of Genes and Genomes bubble diagram of the differentially expressed genes; D: Heatmap of differentially expressed genes; E: RNA-seq results of UNC-51-like kinase 1; F-H: Consistency of the western blotting, quantitative real-time PCR, and RNA-seq results. Values are shown as mean ± SD (n = 4). ^aP < 0.05, and ^bP < 0.01. KEGG: Kyoto Encyclopedia of Genes and Genomes; ULK1: UNC-51-like kinase 1; Con: Control; NC: Negative control; sh: Small hairpin; MARK4: Microtubule affinity-regulating kinase 4; GAPDH: Glyceraldehyde 3-phosphate dehydrogenase; HG: High glucose.