Microtubule affinity-regulating kinase 4 exacerbates diabetic cardiomyopathy by inhibiting UNC-51-like kinase 1-mediated mitochondrial autophagy

Figure 3 Impact of microtubule affinity-regulating kinase 4 knockdown on myocardial injury and fibrosis, blood glucose, body weight, and cardiac function and structure in diabetic cardiomyopathy model mice. A: Hematoxylin and eosin staining of left ventricular tissue (× 200); B: Masson’s staining of left ventricular tissue (× 200); C: Blood glucose levels; D: Body weights; E: The left ventricular ejection fractions; F: The left ventricular short axis shortening rates; G: Left ventricular end-diastolic diameters; H: Left ventricular end-systolic diameters. Values are mean ± SD (n = 4). ^aP < 0.05, and ^bP < 0.01. HE: Hematoxylin and eosin; Con: Control; DCM: Diabetic cardiomyopathy; MARK4: Microtubule affinity-regulating kinase 4; sh: Small hairpin; NC: Negative control; LVEF: Left ventricular ejection fractions; LVFS: Left ventricular shortening fraction; LVIDd: Left ventricular end-diastolic diameters; LVIDs: Left ventricular end-systolic diameter; AAV9: Adeno-associated virus 9.