Microtubule affinity-regulating kinase 4 exacerbates diabetic cardiomyopathy by inhibiting UNC-51-like kinase 1-mediated mitochondrial autophagy

Figure 2 Effect of microtubule affinity-regulating kinase 4 knockdown on mitophagy in mice with diabetic cardiomyopathy. A and B: Evaluation of microtubule affinity-regulating kinase 4 knockdown efficiency in diabetic cardiomyopathy model mice via western blotting quantification; C-G: Western blotting quantification of p62, Parkin, Beclin1, and microtubule-associated protein 1 light chain 3B. Values are mean ± SD (n = 4). ^aP < 0.05, and ^bP < 0.01. DCM: Diabetic cardiomyopathy; MARK4: Microtubule affinity-regulating kinase 4; GAPDH: Glyceraldehyde 3-phosphate dehydrogenase; sh: Small hairpin; Con: Control; NC: Negative control; LC3B: Microtubule-associated protein 1 light chain 3B; AAV9: Adeno-associated virus 9.