Endothelial cell-derived exosomes inhibit high glucose-induced osteoblast ferroptosis by activating microRNA-335-3p/prostaglandin endoperoxide synthase 2

Figure 5 Inhibition of microRNA-335-3p abolishes endothelial cell-exosomes-mediated protection against high glucose-induced oxidative stress in osteoblasts. A: Detection of microRNA-335-3p levels in osteoblasts induced by high glucose after inhibition of microRNA-335-3p by reverse transcription-quantitative polymerase chain reaction; B-D: Results of reactive oxygen species, malondialdehyde and glutathione quantitative analysis; E: Detection of cellular reactive oxygen species levels in each group using dichlorofluorescein fluorescence probe. The bars indicate the mean ± SD from three independent experiments (n = 3). ^aP < 0.05 vs control, ^bP < 0.01 vs control, ^cP < 0.01 vs high glucose, ^dP < 0.01 vs high glucose + exosomes + negative control. Con: Osteoblasts treated with normal glucose; HG: Osteoblasts treated with high glucose; HG + Exos: High glucose + endothelial cell-exosomes; HG + Exos + NC: High glucose + negative control transfection + endothelial cell-exosomes; HG + Exos + inhibitor: High glucose + microRNA-335-3p inhibitor + endothelial cell-exosomes.