Endothelial cell-derived exosomes inhibit high glucose-induced osteoblast ferroptosis by activating microRNA-335-3p/prostaglandin endoperoxide synthase 2

Figure 3 High glucose + exosomes and high glucose group microRNAs differential expression analysis. A: Heatmap of differentially expressed microRNAs (miRs), red to blue represents expression levels from high to low; B: Volcano plot of differentially expressed miRs. Orange dots represent up regulated miRs, green dots represent down regulated miRs, gray dots represent no difference; C: Validation of the top 10 miRs that were significantly upregulated in high glucose (HG) + exosomes (Exos) compared to HG expression, ^aP < 0.05 vs high glucose; D: Differences in miR-335-3p expression among Exos, HG group, and HG + Exos group; E: Interaction diagram of miR with target proteins; F: Kyoto Encyclopedia of Genes and Genomes pathway analysis diagram. The bars indicate the mean ± SD from three independent experiments (n = 3). ^bP < 0.05 vs high glucose, ^cP < 0.05 vs high glucose + exosomes. Con: Osteoblasts treated with normal glucose; HG: Osteoblasts treated with high glucose; HG + Exos: High glucose + endothelial cell-exosomes; miR: MicroRNA; Exos: Exosomes.