Farrerol ameliorates diabetic cardiomyopathy by inhibiting ferroptosis via miR-29b-3p/SIRT1 signaling pathway in endothelial cells

Figure 8 Farrerol inhibits high glucose and free fatty acid-induced ferroptosis via inhibition of miR-29b-3p in endothelial cells. A-D: Protein expression of SIRT1, GPX4 and xCT were detected in endothelial cells (ECs) upon high glucose and free fatty acid, together with Farrerol or miR-29b-3p (n = 3); E and F: Representative immunofluorescence images of SIRT1 in ECs from indicated group. Bar = 50 μm. n = 6; G-H: Flow cytometry is used to detect the changes in lipid peroxidation levels in ECs under different treatment conditions (n = 6). ^aP < 0.05 vs NC; ^bP < 0.05 vs HG/FFA + mimic NC; ^cP < 0.05 vs HG/FFA + miR-29b-3p mimic; ^dP < 0.05 vs HG/FFA + FA + mimic NC. HG/FFA: High glucose and free fatty acid; FA: Farrerol.