Farrerol ameliorates diabetic cardiomyopathy by inhibiting ferroptosis via miR-29b-3p/SIRT1 signaling pathway in endothelial cells

Figure 7 The effects of upregulation or downregulation of miR-29b-3p on cardiac microvascular injury in diabetic cardiomyopathy mice. A and B: Cardiac microvascular density was determined using CD31-positive endothelial cells, and large vessels were excluded (n = 6); C: Perivascular leakage of albumin was detected by immunofluorescence staining and quantified. Bar = 50 μm; D-G: Protein expression of intercellular adhesion molecule-1, VE-cadherin and endothelial nitric oxide synthase were detected by western blot and quantified (n = 3). ^aP < 0.05 vs NC; ^bP < 0.05 vs DCM. DCM: Diabetic cardiomyopathy; ECs: Endothelial cells; ICAM-1: Intercellular adhesion molecule-1; eNOS: Endothelial nitric oxide synthase.