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©The Author(s) 2025.
World J Diabetes. Sep 15, 2025; 16(9): 107663
Published online Sep 15, 2025. doi: 10.4239/wjd.v16.i9.107663
Figure 1
Figure 1 The experimental workflow of spatial transcriptomics in diabetic kidney disease. Kidney tissue is first sectioned and stained with hematoxylin and eosin to retain histological context. Prepared slides undergo spatial barcoding and cDNA synthesis, followed by library construction and sequencing. The resulting data are used to reconstruct spatial gene expression patterns, identify cellular neighborhoods, and infer cell–cell interactions within intact renal tissue.


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