Troxerutin improves diabetic cognitive dysfunction by inhibiting mitochondrial fission mediated by transient receptor potential melastatin 7/calcineurin/dynamin-related protein 1ser637

Figure 7 Silencing transient receptor potential melastatin 7 inhibited expressions of calcineurin and apoptosis-associated molecules, increased expressions of adenosine triphosphate and p-dynamin-related protein 1^ser637 in hippocampal neuronal cells. Cells were preincubated with transient receptor potential melastatin 7 (TRPM7) siRNA or control siRNA for 24 hours and then incubated with high-concentration glucose for 48 hours. A: The normalized mRNA expression of TRPM7, calcineurin, and p-Drp1^ser637; B: The relative protein expression of TRPM7, calcineurin, and p-Drp1^ser637; C: Relative of adenosine triphosphate level; D: The normalized mRNA expression of caspase-3, B-cell lymphoma 2 (Bcl-2)-associated X protein (Bax), and Bcl-2; E: The relative protein expression of the relative protein expression of caspase-3, Bcl-2-associated X protein, and Bcl-2. ^aP < 0.05, compared with normal control + small interfering RNA empty vector group. Data presented as mean ± SEM. Drp1: Dynamin-related protein 1; TRPM7: Transient receptor potential melastatin 7; CaN: Calcineurin; NC: Normal control; siRNA: Small interfering RNA; Bax: B-cell lymphoma 2-associated X protein; Bcl-2: B-cell lymphoma 2.