Troxerutin improves diabetic cognitive dysfunction by inhibiting mitochondrial fission mediated by transient receptor potential melastatin 7/calcineurin/dynamin-related protein 1ser637

Figure 3 The level of transient receptor potential melastatin 7 was increased in diabetic cognitive dysfunction mice and hippocampal neuronal cells dealed with high-concentration glucose. A: The normalized mRNA expression of transient receptor potential melastatin 7 (TRPM7) in the hippocampal tissue; B: The relative protein expression of TRPM7 in the hippocampal tissue; C: Immunofluorescent staining of TRPM7 in the hippocampal tissue; D: The normalized mRNA expression of TRPM7 in high-concentration glucose-treated hippocampal neuronal cells; E: The relative protein expression of TRPM7 in high-concentration glucose-treated hippocampal neuronal cells. ^aP < 0.05, compared with dm group or normal control group. Data presented as mean ± SEM. dm: Control group; db: Diabetes group; NC: Normal control; HG: Hypertonic group (100 mmol/L glucose); DAPI: 4’,6-diamidino-2-phenylindole; TRPM7: Transient receptor potential melastatin 7.