Examining gut microbiota and metabolites to clarify mechanisms of Dimocarpus longan Lour leaf components against type 2 diabetes

Figure 6 Multivariate statistical analysis (n = 6). A: Principal component analysis (PCA) score plot (positive ion mode); B: PCA score plot (negative ion mode); C and D: Orthogonal partial least squares - discriminant analysis (OPLS-DA) score plot (positive ion mode); E and F: OPLS-DA score plot (negative ion mode); G and H: OPLS-DA model validation in positive ion mode via permutation test (n = 200); I and J: OPLS-DA model validation in negative ion mode via permutation test (n = 200). R²Y (model interpretability) and Q²Y (predictive power) are critical metrics for OPLS-DA. A robust model is confirmed when R²Y > Q²Y. Con: Control group; Mod: The model group; Met: The metformin group; HDLC: The high-dose group.