Mechanism of trypsin-mediated differentiation of pancreatic progenitor cells into functional islet-like clusters

Figure 4 E-cadherin effects on phosphorylation of αE-catenin (Ser655/T658) mediated by trypsin. A: Representative western blot images reflecting protein expression of E-cadherin of cells with knockout (KO) of E-cadherin gene (CDH1); B: Representative western blot images reflecting protein expression of phospho-αE-catenin (p-αE-catenin) (Ser655/Thr658) in CDH1 KO cells and difference in trypsin-mediated αE-catenin (Ser655/Thr658) phosphorylation before and after KO; C: Representative western blot images reflecting protein expression of p-αE-catenin (Ser655/Thr658) of restoration of E-cadherin in CDH1 KO cells and difference in trypsin-mediated αE-catenin (Ser655/Thr658) phosphorylation before and after restoration (n = 2). ^aP < 0.05. ^cP < 0.001. P calculated vs control (CON). GAPDH: Glyceraldehyde-3-phosphate dehydrogenase.