Mechanism of trypsin-mediated differentiation of pancreatic progenitor cells into functional islet-like clusters

Figure 3 Phosphorylation of αE-catenin at Ser655/Thr658 during human pancreatic cancer cell line-1 differentiation and effects of casein kinase-1 inhibitor D4476 on human pancreatic cancer cell line-1. A: Representative western blot images reflecting protein expression of phospho-αE-catenin (p-αE-catenin) (Ser655/Thr658) at different time points during human pancreatic cancer cell line-1 (PANC-1) differentiation; B: Statistical analyses of the gray value of western blot images using ImageJ and GraphPad Prism, reflecting the differences in expression of p-αE-catenin (Ser655/Thr658); C: Chemical structure of D4476, inhibitor of casein kinase-1; D: Representative images of PANC-1 cells treated with 2, 5, or 10 μM D4476 for 72 hours, magnification 10 ×; E: Western blot images showed expression of p-αE-catenin (Ser655/Thr658) of PANC-1 cells treated with 2 μM D4476; F: Western blot images showed expression of p-αE-catenin (Ser655/Thr658) of PANC-1 cells treated with 2 μM D4476 at different times; G: Representative images of PANC-1 after 72 hours treatment with 2 μM D4476 at different time points after digestion with trypsin, magnification 10 ×. ^aP < 0.05. ^bP < 0.01. P calculated vs control (CON). GAPDH: Glyceraldehyde-3-phosphate dehydrogenase.