Mechanism of trypsin-mediated differentiation of pancreatic progenitor cells into functional islet-like clusters

Figure 2 αE-catenin is related to trypsin-mediated human pancreatic cancer cell line-1 aggregation and differentiation. A: Expression of αE-catenin protein was detected by western blotting, reflecting knockout (KO) efficiency of catenin alpha 1 (CTNNA1); B: Representative images of KO cells at five time points after digestion with trypsin before and after CTNNA1 KO, magnification 10 ×; C: Representative images of diphenylterazine (DTZ) staining of cell clusters formed at 96 hours before and after KO of CTNNA1, magnification 10 ×. CON: Control.