Mechanism of trypsin-mediated differentiation of pancreatic progenitor cells into functional islet-like clusters

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Jun 15, 2025 (publication date) through Jun 16, 2025

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World Journal of Diabetes

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1948-9358

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Diabetes. Jun 15, 2025; 16(6): 102727
Published online Jun 15, 2025. doi: 10.4239/wjd.v16.i6.102727

Table 4 Single-guide RNA for knockout

Name	Sequences
sgRNA-F2RL1-F	5’-caccgGTCTGCTTCACGACATACAA-3’
sgRNA-F2RL1-R	5’-aaacCTTGTATGTCGTGAAGCAGACc-3’
sgRNA-CDH1-F	5’-caccgCTACGGTTTCATAACCCACA-3’
sgRNA-CDH1-R	5’-aaacTGTGGGTTATGAAACCGTAGc-3’

CDH1: E cadherin; F2RL1: F2R like trypsin receptor 1; SgRNA: Single-guide RNA.

Citation: Gao L, Lai JS, Chen H, Qian LX, Hong WJ, Li LC. Mechanism of trypsin-mediated differentiation of pancreatic progenitor cells into functional islet-like clusters. World J Diabetes 2025; 16(6): 102727
URL: https://www.wjgnet.com/1948-9358/full/v16/i6/102727.htm
DOI: https://dx.doi.org/10.4239/wjd.v16.i6.102727