Basic Study
Copyright ©The Author(s) 2025.
World J Diabetes. Jun 15, 2025; 16(6): 102727
Published online Jun 15, 2025. doi: 10.4239/wjd.v16.i6.102727
Table 2 Polymerase chain reaction procedure
Step
Temperature (°C)
Time
Number of cycles
Initial denaturation945 minutes1
Denaturation9430 seconds35
Annealing50-6030 seconds
Extension7230 seconds/kb
Final extension7210 minutes1
Cooling or holding41