Copyright
©The Author(s) 2025.
World J Diabetes. Jun 15, 2025; 16(6): 102727
Published online Jun 15, 2025. doi: 10.4239/wjd.v16.i6.102727
Published online Jun 15, 2025. doi: 10.4239/wjd.v16.i6.102727
Table 2 Polymerase chain reaction procedure
Step | Temperature (°C) | Time | Number of cycles |
Initial denaturation | 94 | 5 minutes | 1 |
Denaturation | 94 | 30 seconds | 35 |
Annealing | 50-60 | 30 seconds | |
Extension | 72 | 30 seconds/kb | |
Final extension | 72 | 10 minutes | 1 |
Cooling or holding | 4 | ∞ | 1 |
- Citation: Gao L, Lai JS, Chen H, Qian LX, Hong WJ, Li LC. Mechanism of trypsin-mediated differentiation of pancreatic progenitor cells into functional islet-like clusters. World J Diabetes 2025; 16(6): 102727
- URL: https://www.wjgnet.com/1948-9358/full/v16/i6/102727.htm
- DOI: https://dx.doi.org/10.4239/wjd.v16.i6.102727