Neurophysiological mechanisms of electroacupuncture in regulating pancreatic function and adipose tissue expansion

Figure 6 TRPV1 knockout preserves immunometabolic homeostasis in peripancreatic adipose tissue and pancreas. A-F: Impact of electroacupuncture (EA) on the levels of insulin receptor, EGF-like module-containing mucin-like hormone receptor-like 1 (F4/80), interleukin (IL)-10, IL-1β, and insulin in peripancreatic adipose tissue following TRPV1 knockout; G-I: Influence of EA on the expression levels of TRPV1 and calcitonin gene-related peptide-receptor component protein (CGRP) in the pancreas. Vinculin served as loading controls; J: Representative immunofluorescence images of co-expression of TRPV1 and CGRP in the islets of the ST25 group (200 × magnification). Nuclei were stained with 4',6-diamidino-2-phenylindole (blue), TRPV1 is represented by red immunofluorescence, and CGRP by green immunofluorescence; K: Correlation between TRPV1 and CGRP expression as revealed by quantitative analysis of the co-localization of TRPV1 and CGRP immunofluorescence. ^aP < 0.05 compared to the normal control group; ^bP < 0.05 compared to the model or another treatment group. IL: Interleukin; EA: Electroacupuncture