Liraglutide alleviates diabetic cardiomyopathy in streptozotocin-induced diabetic rats by enhancing mitophagy mediated by the AMPK-Parkin signaling pathway

Figure 5 Liraglutide attenuates mitophagy by activating AMPK-Parkin pathway in neonatal rat cardiomyocytes induced by high glucose + palmitic acid. A: Representative colocalization images of lysosomes (LysoTracker Green) and mitochondria (Mito Tracker Red; scale bar: 10 µm); B: Percentage of cells with lysosome (LysoTracker Green) and mitochondria (Mito Tracker Red) colocalization; C: Representative western blotting images of LC3 I, LC3 II, PTEN-induced putative kinase 1 (PINK1) and Pakin in the four groups; D-F: Quantification of the expression of the proteins including LC3 I and LC3 II, PINK1 and Pakin in C; G: Representative western blotting images of p-AMPKα and AMPKα in the four groups; H: Quantification of the expression levels of p-AMPKα and AMPKα in G. Values are presented as the mean ± SD. ^aP < 0.05; ^bP < 0.001. Con: Control group; HG: High glucose; PA: Palmitic acid.