RRM2 attenuates the renal tubular ferroptosis in diabetic kidney disease through PI3K/Akt/Nrf2 pathway

Figure 6 Ribonucleotide reductase regulatory subunit M2 overexpression modulates ferroptosis-related indicators and proteins through the PI3K/Akt pathway. A: Colorimetric method was used to analyze the glutathione content; B: Cystine uptake capacity was measured in high glucose (HG)-treated HK-2 cells; C: The concentration of Fe²⁺ was measured in the HG-treated HK-2 cells; D: Western blotting was performed to determine ferroptosis-related proteins; E-H: Nrf2 (E; nuclear, normalized to histone), SLC7A11 (F) GPX4 (G), and quantification of Nrf2 (H; total, normalized to GAPDH). Data are presented as mean ± SD in triplicate and analyzed using one-way ANOVA. ^aP < 0.001 vs control group; ^bP < 0.001 vs HG group; ^cP < 0.001 vs HG + overexpressing-ribonucleotide reductase regulatory subunit M2 group. HG: High glucose; OE-RRM2: Overexpressing ribonucleotide reductase regulatory subunit M2; GSH: Glutathione.