RRM2 attenuates the renal tubular ferroptosis in diabetic kidney disease through PI3K/Akt/Nrf2 pathway

Figure 5 Ribonucleotide reductase regulatory subunit M2 overexpression suppresses ferroptosis by activating the PI3K/Akt pathway. A: HK-2 cells were stained with Mito FerroGreen to visualize mitochondrial ferrous iron using green fluorescence. A representative image of FerroGreen-positive cells is shown (200 × magnification); B: Quantification of the percentage of FerroGreen-positive cells relative to DAPI-positive cells. Data are presented as mean ± SD in triplicate and analyzed using one-way ANOVA. ^aP < 0.001 vs control group; ^bP < 0.001 vs high glucose (HG) group; ^cP < 0.001 vs HG + OE- ribonucleotide reductase regulatory subunit M2 group. HG: High glucose; OE-RRM2: Overexpressing ribonucleotide reductase regulatory subunit M2.