N7-methylguanosine-related gene decapping scavenger enzymes as a novel biomarker regulating epithelial cell function in diabetic foot ulcers

Figure 6 Validation of decapping scavenger enzyme expression in skin wounds. A: Decapping scavenger enzyme (DCPS) mRNA expression decreased significantly at 6 hours post-injury and gradually recovered during wound healing; B and C: DCPS expression was reduced in skin tissues of both diabetic foot ulcer patients and diabetic mice at the RNA level, n = 3; D and E: Immunofluorescence analysis revealed reduced DCPS (red) expression in human diabetic skin tissues, n = 3 (scale bar: 400 μm); F and G: Immunofluorescence revealed reduced DCPS (red) expression in diabetic mouse skin tissues, n = 3 (scale bar: 400 μm). Statistical analyses were performed using GraphPad Prism 8. The results were expressed as mean ± SD. DFU: Diabetic foot ulcer; DCPS: Decapping scavenger enzyme; DM: Diabetes mellitus; NC: Negative control. ^aP < 0.001, and ^bP < 0.01.