Exosomal transfer of miR-375-3p from pancreatic β cells to hepatocytes impairs hepatic glycogenesis via Rbpj repression

Figure 5 miR-375-3p inhibition abrogates the influence of glucotoxic pancreatic cell-derived exosomes on hepatocytes. Hep1-6 cells were transfected with miR-375-3p mimics or control mimics for 48 hours using RNAi Max. A-C: Western blot analysis of AKT/GSK signaling and quantification of phosphorylated proteins; D: Glycogen content analysis in Hep1-6 cells. Hep1-6 cells were transfected with a miR-375-3p inhibitor or control inhibitor for 48 hours and treated with exosomes from low- or high-glucose-stimulated MIN-6 cells for 24 hours; E and F: Western blot analysis of AKT/GSK signaling and Rbpj protein levels; G: Densitometric quantification of the relative protein levels normalized to β-actin performed using ImageJ; H: Glycogen content analysis in Hep1-6 cells. The experiments were repeated three times. ^aP < 0.05, ^cP < 0.001. Western blot raw data in Supplementary material.