Exosomal transfer of miR-375-3p from pancreatic β cells to hepatocytes impairs hepatic glycogenesis via Rbpj repression

Figure 4 miR-375-3p is transferred from glucotoxic pancreatic cells to hepatocytes via exosomes. Relative miR-375-3p levels were quantified by quantitative reverse transcription polymerase chain reaction. A: Hep1-6 and MIN-6 cells after low- or high-glucose stimulation; B: Pancreatic and liver tissues of high-fat diet (HFD)-fed or chow diet (CD)-fed mice; C: Primary islet cells (IPCs) and LPCs from HFD-fed or CD-fed mice; D: Hep1-6 cells treated with conditioned media from IPCs (IPC-Sup); E: Hep1-6 cells treated with conditioned media from MIN-6 cells (MIN6-Sup); F: Hep1-6 cells cocultured with MIN-6 cells using Transwell plates (MIN6-Transwell); G: Plasma of HFD-fed or CD-fed mice; H-J: Exosomes or exosome-depleted supernatantsisolated from pancreatic tissues (pancreas-Exos), IPCs (IPC-Exos), and MIN-6 cells (MIN-6 Exos); K: Hep1-6 cells treatment with conditioned media from GW4869-treated MIN-6 cells. The experiments were repeated three times. ^aP < 0.05, ^bP < 0.01, ^cP < 0.001.