Exosomal transfer of miR-375-3p from pancreatic β cells to hepatocytes impairs hepatic glycogenesis via Rbpj repression

Figure 2 Supernatants of glucotoxic pancreatic cells impair glycogenesis of hepatocytes. MIN-6 cells were treated with low (5.5 mmol/L) or high (33.5 mmol/L) glucose. A: Cell viability was detected by MTT assay; B and C: TUNEL staining and quantification of apoptotic MIN-6 cells; D: Insulin mRNA expression levels in MIN-6 cells. Hep1-6 cells were treated with supernatant from MIN-6 cells (MIN6-Sup); E-G: Western blot analysis of AKT and GSK-3β phosphorylation levels; H: Detection of the glycogen content. Hep1-6 cells were treated with conditioned media from primary islet cells (IPC-Sup) of high-fat diet-fed or chow diet-fed mice; I-K: Western blot analysis of AKT and GSK-3β phosphorylation levels; L: Detection of the glycogen content. MIN-6 and Hep1-6 cells were cocultured using Transwell plates; M-O: Western blot analysis of AKT and GSK-3β phosphorylation levels; P: Detection of the glycogen content. The experiments were repeated three times. ^aP < 0.05, ^bP < 0.01, ^cP < 0.001. Western blot raw data in Supplementary material.